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KMID : 0371320010600010029
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Journal of the Korean Surgical Society
2001 Volume.60 No. 1 p.29 ~ p.35
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Detection of Axillary Lymph Node Micrometastases in Breast Cancer Using RT-PCR Comparison the Results of MUC1, Cytokeratin 19
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Lee Ryung-Ah
Oh Seung-Keun
Choi Kook-Jin
Youn Yeo-Kyu
Roh Dong-Young
Kim Sung-Won
Kang Hee-Joon
Kang Han-Sung
Kim Seong-Suk
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Abstract
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Purpose: The detection of axillary lymph-node micrometastases in breast-cancer patients by using the reverse transcriptase-polymerase chain reaction (RT-PCR) may provide a good guide for postoperative therapy. To evaluate effectiveness of RT-PCR for the detection of micrometastasis, we have compared the results of conventional, immunohistochemical (IHC) staining with those of RT-PCR.
Methods: We conducted RT-PCR amplifications of MUC1 and cytokeratin (CK) 19 on lymph nodes from 40 breast- cancer patients and conducted IHC staining for cytokeratin. The results of histological examination and cytokeratin IHC staining were compared with the RT-PCR results for the detection of lymph-node micrometastases.
Results: Nineteen of 40 cases (47.5%) were lymph-node positive and twenty-one cases (52.5%) were lymph-node negative. Using RT-PCR MUC1 and CK19 expressions were detected in all positive lymph nodes and in 4 (19.0%) and 5 (23.8%), in negative nodes, respectively. Not all positive nodes were stained by IHC (94.7%), and none of the negative lymph nodes were stained by IHC. Thus, IHC staining was ineffective in detecting micrometastases.
Conclusion: Detection of MUC1 and CK19 by using RT- PCR can be a more accurate and useful method than IHC staining for the detection of axillary lymph-node micro-metastases in breast cancer.
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KEYWORD
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Breast cancer, Lymph node micrometastases, MUC1, Cytokeratin 19, RT-PCR
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